1 Lusamine (Pokemon Sun and Moon)
Lusamine is a fictional character introduced in Pokémon Sun and Moon. She is the head of the Aether Foundation.
Oh, she's DEFINITELY my favorite female video game villain as of right now. - ModernSpongeBobSucks
She look like an cool girl!
Reminds me of handsome jack a misunderstod person where her children betrey her and she trys to get power by relying on a Non human power and is also some what of a psychotic person
She defends on her's nihilego? or training her's nihilego? training.
2 Mother Brain (Metroid series)
3 GLaDOS (Portal series)
4 Mileena (Mortal Kombat series)
A very sick female
5 Kreia (Star Wars Knights of the Old Republic II: The Sith Lords)
Master of deception and manipulation with deep knowledge of the sith and the jedi.
Great villain and character. And the game is really good.
6 The Boss (Metal Gear Solid 3: Snake Eater)
She is NOT a villain if you've seen the ending. - HeavyDonkeyKong
7 Harley Quinn (Batman: Arkham series)
Harley Quinn is a fictional character appearing in American comic books published by DC Comics, commonly as a sidekick of the Joker.
8 Shodan (System Shock series)
9 Queen Sectonia (Kirby: Triple Deluxe)
10 Sarah Kerrigan (StarCraft series)
11 Gruntilda (Banjo-Kazooie)
12 Dark Queen (Battletoads)
13 Jacqueline Natla (Tomb Raider series)
14 Mui Lin (Rise to Honor)
15 Sniper Wolf (Metal Gear Solid / Metal Gear Solid: The Twin Snakes)
One of the best Metal Gear characters and in video games overall. Great backstory to the character and I love the look of her. Menacing and compassionate.
16 Alma (Ninja Gaiden 2004)
17 Cia (Hyrule Warriors)
18 Eve (Parasite Eve)
My favorite villain! - MeeMeeCandy777
19 Alphys (Undertale)
Alphys is an adorably dorky, lab-coat-wearing, bespectacled, yellow-skinned lizard scientist who resides in her lab in Hotland in the critically acclaimed cult classic RPG known as Undertale, wherein she partakes in the daily act of watching insane amounts of anime and having a vast majority of the ...read more.
20 Katherine Marlowe (Uncharted 3: Drake's Deception)
21 Chara (Undertale)
Chara is a character in the game Undertale by Toby "Radiation" Fox who is one of the seven people to fall to the Underground prior to the fall of the player character. His name can be changed by the player and will be assumed as the player character, until the Pacifist Route when Asriel asks for the ...read more.
22 Alexia Ashford (Resident Evil Code: Veronica)
23 Junko Enoshima (Dangan Ronpa)
24 Sorceress (Spyro: Year of the Dragon)
25 Jeane (No More Heroes)
26 Bad Girl (No More Heroes)
27 Mistral (Metal Gear Rising: Revengeance)
28 Dark Samus (Metroid Prime Series)
29 Sofia Lamb (Bioshock 2)
30 Queen Myrrah (Gears of War Series)
31 Excella Gionne (Resident Evil 5)
32 Poison Ivy (Batman: Arkham Series)
Poison Ivy is a fictional supervillain appearing in American comic books published by DC Comics, commonly as an adversary of the superhero Batman.
33 Courtney Gears (Ratchet & Clank 3: Up Your Arsenal)
34 Mother Hydra (Call of Cthulhu: Dark Corners of the Earth)
35 Queen Slug-for-a-butt (Earthworm Jim)
36 Maia Acheron (Jak and Daxter: The Precursor Legacy)
37 Cassandra De Vries (Perfect Dark)
38 Jo Slade (Dead Rising)
39 Mercy (The Incredible Hulk: Ultimate Destruction)
40 Kalus (Kameo: Elements of Power)
41 Mariska (Lollipop Chainsaw)
42 Ultimecia (Final Fantasy VIII)
43 Colonel Zarpedon (Borderlands: The Pre-Sequel)
44 Nadine Ross (Uncharted 4: a Thief's End)
45 Elizabeth Greene (Prototype)
46 Laura Victoriano (The Evil Within)
47 Chaos Witch Quelaag (Dark Souls)
48 Brooke Augustine (Infamous: Second Son)
49 Mytha, the Baneful Queen (Dark Souls II)
50 Lost Sinner (Dark Souls II)