1 Lusamine (Pokemon Sun and Moon)
Lusamine is a fictional character introduced in Pokémon Sun and Moon. She is the head of the Aether Foundation.
Oh, she's DEFINITELY my favorite female video game villain as of right now. - ModernSpongeBobSucks
She look like an cool girl!
Reminds me of handsome jack a misunderstod person where her children betrey her and she trys to get power by relying on a Non human power and is also some what of a psychotic person
She defends on her's nihilego? or training her's nihilego? training.
2 Mileena (Mortal Kombat series)
Mileena is a character from the Mortal Kombat franchise who debuted in the sequel Mortal Kombat II as a clone of Kitana by Shang Tsung using Tarkatan DNA resulting in her mouth having razor sharp teeth that of a Tarkatan like Baraka, she hated her sister Kitana and vowed to destroy her and take over ...read more.
A very sick female
Just the best
3 GLaDOS (Portal series)
4 Harley Quinn (Batman: Arkham series)
Harley Quinn is a fictional character appearing in American comic books published by DC Comics, commonly as a sidekick of the Joker.
5 Mother Brain (Metroid series)
I didn't know she even had a gender.
6 Kreia (Star Wars Knights of the Old Republic II: The Sith Lords)
Master of deception and manipulation with deep knowledge of the sith and the jedi.
Great villain and character. And the game is really good.
7 The Boss (Metal Gear Solid 3: Snake Eater)
She is NOT a villain if you've seen the ending. - HeavyDonkeyKong
8 Shodan (System Shock series)
9 Sarah Kerrigan (StarCraft series)
Responsible for millions of deaths, strong, sexy, weird and powerful an Adult villian not an Anime baby villian harming Pokemons or something. The Queen of Blades, she will slaughter your planet how is she number 10 smfh
10 Queen Sectonia (Kirby: Triple Deluxe)
She Reminds me of Lusamine from Sun and Moon God I wish
I love her boss track and I'm big fan of Queen Sectonia she is my favorite Kirby Villain I wish she'll be in Smash Ultimate with Jibanyan, Crash, Sora, Spyro & 2 of Jibanyan's Echo fighters Komasan and Usapyon
11 Mz. Ruby (Sly Cooper)
12 Contessa (Sly 2)
13 Courtney Gears (Ratchet & Clank 3: Up Your Arsenal)
14 Sniper Wolf (Metal Gear Solid / Metal Gear Solid: The Twin Snakes)
One of the best Metal Gear characters and in video games overall. Great backstory to the character and I love the look of her. Menacing and compassionate.
15 Gruntilda (Banjo-Kazooie)
Gruntilda Winkybunion often referred to as "Grunty" is the main antagonist of the Banjo-Kazooie series debuting in the first Banjo-Kazooie game in 1998.
16 Jacqueline Natla (Tomb Raider series)
17 Dark Queen (Battletoads)
18 Poison Ivy (Batman: Arkham Series)
Poison Ivy is a fictional supervillain appearing in American comic books published by DC Comics, commonly as an adversary of the superhero Batman.
19 Maia Acheron (Jak and Daxter: The Precursor Legacy)
20 Mui Lin (Rise to Honor)
21 Alma (Ninja Gaiden 2004)
22 Cia (Hyrule Warriors)
23 Eve (Parasite Eve)
My favorite villain! - MeeMeeCandy777
24 Queen Slug-for-a-butt (Earthworm Jim)
25 Junko Enoshima (Dangan Ronpa)
Junko Enoshima (江ノ島 盾子 Enoshima Junko), is a student in Hope's Peak Academy's Class 78th, and a participant of the Killing School Life featured in Danganronpa: Trigger Happy Havoc. Her title is Ultimate Fashionista/True Ultimate Despair
She is the queen of despair and will torture every inch of humanity and would do anything just to deepen her own despair
26 Katherine Marlowe (Uncharted 3: Drake's Deception)
27 Sorceress (Spyro: Year of the Dragon)
28 Alexia Ashford (Resident Evil Code: Veronica)
29 Chara (Undertale)
Chara is a character in the game Undertale by Toby "Radiation" Fox who is one of the seven people to fall to the Underground prior to the fall of the player character. Her name can be changed by the player and will be assumed as the player character, until the Pacifist Route when Asriel asks for the ...read more.
30 Excella Gionne (Resident Evil 5)
She's so sexy
31 Ultimecia (Final Fantasy VIII)
32 Laura Victoriano (The Evil Within)
33 Jeane (No More Heroes)
34 Bad Girl (No More Heroes)
35 Mistral (Metal Gear Rising: Revengeance)
36 Dark Samus (Metroid Prime Series)
37 Sofia Lamb (Bioshock 2)
38 Queen Myrrah (Gears of War Series)
39 Mother Hydra (Call of Cthulhu: Dark Corners of the Earth)
40 Cassandra De Vries (Perfect Dark)
41 Alphys (Undertale)
Alphys is an adorably dorky, lab-coat-wearing, bespectacled, yellow-skinned lizard scientist who resides in her lab in Hotland in the critically acclaimed cult classic RPG known as Undertale, wherein she partakes in the daily act of watching insane amounts of anime and having a vast majority of the ...read more.
42 Jo Slade (Dead Rising)
43 Mercy (The Incredible Hulk: Ultimate Destruction)
44 Kalus (Kameo: Elements of Power)
45 Mariska (Lollipop Chainsaw)
46 Colonel Zarpedon (Borderlands: The Pre-Sequel)
47 Nadine Ross (Uncharted 4: a Thief's End)
48 Elizabeth Greene (Prototype)
49 Chaos Witch Quelaag (Dark Souls)
50 Brooke Augustine (Infamous: Second Son)