1 Alex Louis Armstrong - Fullmetal Alchemist
Alex Louis Armstrong is a fictional character from the Fullmetal Alchemist manga series and its adaptations created by Hiromu Arakawa.
2 Hades - Kid Icarus: Uprising
He basically is a properly evil and even more pompous version of James
3 Stocking Anarchy - Panty & Stocking with Garterbelt
She literally is cuter than her cat, and James literally is cuter than his Meowth
4 Dan Hibiki - Street Fighter
He basically is both a human version of James's Meowth and a more Asian version of James
5 Samus - Metroid
Samus Aran is the protagonist of the Metroid science fiction action-adventure game series by Nintendo.
James (after meeting Samus): I don't think that I am tough enough to be that WOMAN!
Meowth (whose head contains numerous pictures of the Flaming Moltres): YEAH...
6 Narcis Prince - Punch-Out
7 Dr. Frank N. Furter - The Rocky Horror Picture Show
8 C-3PO - Star Wars
9 Edward - Cowboy Bebop
10 Miguel - The Loud House
11 N. Tropy - Crash Bandicoot
James would see N. Tropy flirting with his own female self and then immediately decide to start flirting with HIS own female self
12 Zim - Invader Zim
Zim is the titular protagonist and anti-hero of the American animated series Invader Zim, created by Jhonen Vasquez for Nickelodeon.
13 Sephiroth - Final Fantasy VII
Sephiroth is a fictional character and main antagonist in the role-playing video game Final Fantasy VII developed by Square.
14 Dudley - Street Fighter
15 James Bond - 007
Royal Navy Commander James Bond is a fictional character created by British journalist and novelist Ian Fleming in 1953. He is the protagonist of the James Bond series of novels, films, comics and video games.
16 Captain Falcon - F Zero
Douglas Jay Falcon, known commonly as Captain Falcon, is a playable character in the racing video game series F-Zero.
17 Vega - Street Fighter
Vega, is a fictional character from the Street Fighter fighting game series by Capcom. Vega is a mask-wearing, claw-wielding fighter from Spain who uses a personal fighting style combining Japanese ninjutsu and Spanish bullfighting, earning him the nickname of "Spanish Ninja".
18 Remy - Street Fighter
19 Mr. Garrison - South Park
Herbert Garrison is a recurring character in the American animated television series South Park. He is voiced by Trey Parker.
20 Alucard - Castlevania
21 Timon - The Lion King
22 Prince Adam / He-Man - He-Man
23 Selina Kyle / Catwoman - Batman Returns
24 Byakuya Togami - Danganronpa
Byakuya Togami is a student in Hope's Peak Academy's Class 78th, and a participant of the Killing School Life featured in Danganronpa: Trigger Happy Havoc. His title is Ultimate Affluent Progeny.
25 Garfield - Garfield