1 Stewie (Gay) - Family Guy
2 C-3P0 (Gay) - Star Wars
This list is a load of crap! 3PO is a ROBOT. He doesn't have a physical gender.
3PO is gay? Where'd that idea come from? (Also, he's a protocol droid, mind you) - RiverClanRocks
Don't you know? EVERYBODY'S LGBTQRSTUV now. It's the popular brainwash meme.
3 Mr. Garrison (Gay) - South Park
4 Samus (Trans) - Metroid
5 Nico di Angelo (Gay) - Percy Jackson Series
Nico di Angelo is a fictional character created by Rick Riordan.He appears in Percy Jackson & The Olympians, Hero of Olympus, and parts of Trails of Apollo. As a son of Hades, the boy is a demigod. He befriended Percy and had many roles in his long time appearance including stopping Minos, fighting ...read more.
6 Apollo (Bi) - Percy Jackson Series
7 Alphys (Bi) - Undertale
8 Big Gay Al (Gay) - South Park
9 Cupid (Bi) - Fairly Odd Parents
10 Harley Quinn (Bi) - Batman: the Animated Series
11 Korra (Bi) - The Legend of Korra
Avatar Korra is the title lead character in Nickelodeon's animated television series The Legend of Korra, in which she is depicted as the current incarnation of the Avatar, responsible for maintaining peace and balance in the world.
She could like, step on me - wheelers-
12 Mettaton (Trans) - Undertale
13 Luna Loud (Bi) - The Loud House
How is she not number one Luna’s the best
14 Nanoha Takamachi - Magical Girl Lyrical Nanoha
She's lesbian - PerfectImpulseX
15 Heffer (Gay) - Rocko's Modern Life
16 Music Meister (Gay) - Batman
17 Zonda The Lustful Mirage (Bigender/Pansexual) - Azure Striker Gunvolt
A plug for every socket - PerfectImpulseX
18 Scar (Gay) - The Lion King
We're did this come from?!?!
19 Asami Sato (Bi) - The Legend of Korra
20 Ren Hoek (Gay) - Ren & Stimpy
21 Will Solace (Gay) - Percy Jackson Series
22 Tara Maclay (Lesbian) - Buffy the Vampire Slayer
23 Undyne (Lesbian) - Undertale
24 Waylon Smithers (Gay) - The Simpsons
Smithers is the best.
25 Frisk (Trans) - Undertale
Accidentally Voted For This One Honestly - WWEWBMortalKombatFan
Frisk was specifically made to be androgynous, meaning the pronouns would be they/them. They were made this way so that the player can get a better sense of the role they play in the game.
What the heck is wrong with you people? Frisk is just an ordinary kid. That's it. A KID. - RiverClanRocks
26 Astolfo (Bisexual) - Fate/Apocrypha
"Astolfo attac, Astolfo protec, but most importantly, Astolfo has a dic." - ModernSpongeBobSucks
27 Daisy (Trans) - Super Mario Bros.
Not all females that are masculine are trans. - Callistowo
Where did this idea even come fr om? w here's the proof for this? - nickdoritosyum
28 Timmy's Dad (Gay) - Fairly Odd Parents
29 Hatsuharu Sohma (Bi) - Fruits Basket
30 Principal Willoughby (Gay) - Jimmy Neutron
31 Marceline (Lesbian) - Adventure Time
Marceline the Vampire Queen is a fictional character in the American animated Cartoon Network television series Adventure Time, created by Pendleton Ward.
She had a boyfriend, making her either bi or pan.
32 Rainbow Dash (Lesbian) - Friendship Is Magic
Rainbow Dash is a female Pegasus pony from the popular 2010 kid's show My Little Pony: Friendship is Magic. She represents the element of loyalty and is one of 6 mane characters on the show. ...read more.
Proof? Just because you're a tomboy does not mean you're a lesbian. - RiverClanRocks
33 Finn (Gay) - Adventure Time
34 Tracer (Lesbian) - Overwatch
35 Dio Brando (Bisexual) - JoJo's Bizarre Adventure
KONO DIO DA! - PerfectImpulseX
36 Cure Moonlight / Yuri Tsukikage (Lesbian) - HeartCatch Precure
37 Sailor Uranus (Lesbian) - Sailor Moon
38 Lammy (Lesbian) - Um Jammer Lammy
I want to "huck" her - xandermartin98
39 Shamal Yagami (Lesbian) - Magical Girl Lyrical Nanoha
40 Subaru Nakajima (Lesbian) - Magical Girl Lyrical Nanoha
41 Link (Trans) - The Legend Of Zelda
Ha! Yeah right!
42 Sephiroth (Trans) - Final Fantasy VII
Sephiroth is a fictional character and main antagonist in the role-playing video game Final Fantasy VII developed by Square.
43 Kefka (Gay) - Final Fantasy VI
44 Stimpy (Gay) - Ren & Stimpy
45 Spongebob (Bi) - Spongebob Squarepants
Ok, the creator of SpongeBob specifically said that he is asexual.
46 Sandy (Trans) - Spongebob Squarepants
47 Pyro (Trans) - Team Fortress 2
48 James (Gay) - Pokemon
Where'd you get this from? - RiverClanRocks
Er, no. He just has purple hair. A lot of anime males have purple or pink hair, like Natsu from Fairy Tail. - RiverClanRocks
49 Midna (Trans) - Twilight Princess
The Twilight Princess herself, Midna was transformed into an imp by Zant, who usurped her and turned her subjects into monsters. She set out to retrieve the Fused Shadows so she could take down Zant and save her kingdom.
50 The Chameleon Brothers (Gay) - Rocko's Modern Life