41 Dr. Gregory House (House)
42 Winifred "Fred" Burkle (Angel)
Fred was one of my favorite charactors from angel and even though she didn't work in a lab or have the doctor title doesn't mean she wasn't just as smart. when Fred would go on about physics you could tell that she was scary smart.
43 Rupert Giles (Buffy the Vampire Slayer)
He was smart, but not half as smart as the rest of the people on this list.
44 Wesley Wyndam-Pryce (Buffy the Vampire Slayer)
45 Mohinder Suresh (Heroes)
On heroes he was the smartest. he knew exactly what was happening even nobody else did.. he was the professor that brought everyone together after his fathers' passing.
46 John Locke (Lost)
While, not as smart as the rest. he sure kept everyone on their toes in a tough situation on lost.
47 Sam Puckett (iCarly)
48 Professor Frink (The Simpsons)
How is he not smarter than Lisa? Has anyone seen this show?
49 Dr. Dick Solomon (3rd Rock From The Sun)
50 Jimmy Neutron (The Adventures of Jimmy Neutron: Boy Genius)
James "Jimmy" Isaac Neutron is a fictional character and the main protagonist of the Jimmy Neutron franchise . He is most notably the main protagonist of the 2001 American 3D computer-animated comic science fiction film Jimmy Neutron: Boy Genius and the main protagonist of the American computer animated ...read more.
51 Susan & Mary Test (Johnny Test)
52 Stewie Griffin (Family Guy)
Stewart Gilligan "Stewie" Griffin is a main character from the animated television series Family Guy. Obsessed with violence and matricide, Stewie is the youngest child of Peter and Lois Griffin, and the brother of Meg and Chris Griffin.
Super genius baby invented a time machine can do amazing fight moves and has a whole bunch of guns
53 Twilight Sparkle (My Little Pony: Friendship Is Magic)
Twilight Sparkle is the primary main character of My Little Pony Friendship is Magic. She is a female unicorn pony who transforms into an Alicorn and becomes a princess in Magical Mystery Cure.
Smartest MLP FIM character ever
54 Plankton (SpongeBob SquarePants)
Sheldon James Plankton, or simply Plankton, is a character in the Nickelodeon animated television series SpongeBob SquarePants.
55 Edd (Ed, Edd, N Eddy)
Edd, mostly referred to by his nickname "Double D," is one of the three protagonists in the Canadian-American animated comedy television series Ed, Edd n Eddy. He is the physically weakest but the most intelligent member of the Eds.
56 Mandy (The Grim Adventures Of Billy And Mandy)
Amanda "Mandy" is a fictional character from the American animated series The Grim Adventures of Billy & Mandy created by Maxwell Atoms for Cartoon Network. She is perpetually a cynical, merciless girl from Endsville.
57 Leonardo da Vinci (Da Vinci's Demons)
" The most diversely talented person ever to have lived. "
58 Agent Cooper (Twin Peaks)
She is the one of the three main characters in the show The Powerpuff Girls. She was first created by Craig McCracken in 1992. She has short raven hair and green eyes with a green dress. She is known as the "toughest fighter" for her tomboyish attitude, being aggressive, and her love for fighting crime. ...read more.
60 Rachel Berry (Glee)
Rachel Barbra Berry is a fictional character and de facto female lead from the Fox musical comedy-drama series Glee.