1 Glenn Quagmire
Glenn Quagmire, often referred to as just Quagmire, is a character from the animated television series Family Guy.
Brian had sex with Quagmire's dad
He hates him, so I think it's obvious that he killed Brian
It's not him but he hates Brian
2 Vinny (Family Guy)
No I don't think so by lauren petrovic
3 Ajit Pai
Ajit Pai is the chairman of the FCC. He has became infamous for passing the repeal of net neutrality as of December 14, 2017. He used to be a lawyer for Verizon.
It was definitely Ajit Pai. He loves taking everything away, including net neutrality. - kcianciulli
Ajit Pai is a heartless monster! - kcianciulli
Wow! he's in the top ten now! - kcianciulli
4 Sideshow Bob
Dr. Robert Underdunk Terwilliger, better known as Sideshow Bob, is a recurring character in the animated television series The Simpsons.
I think he killed Brian.
5 Oz (Fanboy and Chum Chum)
6 Penelope (Family Guy)
She tried to kill him once, so this could be her second try.
7 John Kricfalusi
Michael John Kricfalusi /ˌkrɪsfəˈluːsi/, better known as John K., is a Canadian animator, voice actor, producer, writer, and director best known for creating The Ren & Stimpy Show and founding the animation company Spümcø.
Nice going, John K, you made everyone cry! - kcianciulli
Wow, I didn't know John K. was such a monster. First, he molested underaged girls, then it turns out he killed one of my most favorite characters in all media. He also ruined one of the best friendships in all media. I don't think he'd care if he hurt anyone because that's how he is. This man is evil. He needs to be locked up in a mental hospital and or go to prison. Go to Hell, John K.! - kcianciulli
Wow, John K. made it to the top ten! - kcianciulli
I'm betting that it was John K. who drove that car and killed Brian. - kcianciulli
8 Ed, Edd, and Eddy
9 Seth MacFarlane
Seth Woodbury MacFarlane is an American television producer, filmmaker, actor, and singer, working primarily in animation and comedy, as well as live-action and other genres.
Actually this is the guy who killled brian guess why
10 Justin Bieber
Justin Drew Bieber (born March 1, 1994) is a Canadian singer, songwriter, and record producer. He currently resides in Ontario, Canada and is Christian. He is the son of author Pattie Mallette. ...read more.
Well he drank and drove before, so I would not be surprised if he killed Brian. - Murvine_Taylor
11 Tad & Chad (Fairly OddParents)
12 Ariel Castro
13 A Brian from the future
But if he went back in time and killed himself, he wouldn't be alive to go back in time and kill himself.
Perhaps a future Brian made some mistake like the 9/11 thing in Back to the pilot, and realised the only way to stop himself and save the world, was to go back in time and kill himself before the incident happened.
14 Barney the Dinosaur
Barney The Dinosaur is a purple dinosaur from the TV series "Barney and Friends", as well as the VHS series before that known as "Barney and the Backyard Gang". He was created by Sheryl Leach in 1987, to entertain her 2 year old son. He is infamously known for his "I Love You" song, and his TV series ...read more.
15 Christian Weston Chandler
Christian Weston Chandler (Who is now known as Christine Weston Chandler), best known as Chris-chan, is an Autistic man who is the creator of the infamous webcomic Sonichu. He was discovered by trolls in 2007, which lead to him retaliating. In 2011, he came out as a tomgirl and started cross-dressing; ...read more.
God, Chris, Why would you kill Brian? That just makes you even more evil. - kcianciulli
16 Dollarnator (Fanboy and Chum Chum)
17 Eric Cartman
Eric Theodore Cartman is one of the main characters in the animated television series South Park, created by Matt Stone and Trey Parker, and voiced by Trey Parker.
Because he hates Family Guy
He's to young to drive a car
18 Paul Walker
Paul William Walker IV was an American actor . Walker began his career guest-starring in several television shows such as The Young and the Restless and Touched by an Angel .
Don't Insult Him
Who Put Him Here Shall Burn In Hell
19 Bendy (Foster's Home For Imaginary Friends)
Everyone knows it's Bendy. - KalloFox34
This Yellow Bastard hated seeing Brian and Stewie, so the only way to end their friendship was to take Brian out.
Bendy Hated Seeing Brian and Stewie, so he decided to kill Brian.
Trust Me, It Was Bendy.
20 The Annoying Orange
Orange doesn't care if he hurt the Griffin family and the other citizens of Quahog because he has no sympathy! - kcianciulli
He ruined Brian and Stewie's friendship by killing Brian so he can make his motorboat, nya nya nya, and laughing sounds.
Orange Loves seeing people in pain.
Comment If You Think Orange Did It.
22 Roger (American Dad)
He killed Brian by putting mucus on him and he died! 🤣🤣 - Bizcut4life
Roger put his alien mucus on him and he died! 🤣🤣 - Bizcut4life
Aku is a fictional character and the main antagonist of Samurai Jack, an American animated television series created by Genndy Tartakovsky for Cartoon Network.
24 R. Kelly
Robert Sylvester Kelly, known professionally as R. Kelly, is an American recording artist, songwriter, record producer, and former professional basketball player.
25 Amy Schumer
Amy Beth Schumer is an American stand-up comedian, writer, actress, and producer. She is the creator, co-producer, co-writer and star of the sketch comedy series Inside Amy Schumer, which debuted on Comedy Central in 2013 and has received a Peabody Award.
26 Logan Paul
Logan Alexander Paul is an American social media entertainer and actor. He first gained fame through videos shared on the internet video service Vine, in which the athletic Paul engages in physical comedy, including slapstick pratfalls and public splits.
Since he has been getting a lot of flak lately, I wouldn't be surprised if he did it. - kcianciulli
27 Janitor Poopatine (Fanboy and Chum Chum)
28 Bradwurst (Random! Cartoons)
29 Lupe (Fanboy and Chum Chum)
30 Boog (Fanboy and Chum Chum)
31 Kong Li (Three Delivery)
Oroku "The Shredder" Saki is a fictional character in the Teenage Mutant Ninja Turtles comics and all of its related media.
33 Strong Man (Fairly Odd Parents)
34 Lunch Lady Cram (Fanboy and Chum Chum)
35 Sheldon Dinkleberg (Fairly Odd Parents)
Brian's dead! Dinkleberg! P.S. Brian's Alive
36 Mayor West (Family Guy)
37 Homer Simpson
Homer Jay Simpson is the protagonist of the American animated television series The Simpsons as the patriarch of the eponymous family.
38 Kyle (Fanboy and Chum Chum)
39 Mr. Mufflin (Fanboy and Chum Chum)
40 Tarrlok (Legend Of Korra)
41 Adam West
William West Anderson, known professionally as Adam West, was an American actor known primarily for his role as Batman in the 1960s ABC series of the same name and its 1966 theatrical feature film.
42 Tony Sirico
43 Bender (Futurama)
Bender Bending Rodríguez is a fictional character who is one of the main characters in the animated television series Futurama. He was created by series creators Matt Groening and David X. Cohen and is voiced by John DiMaggio.
44 Ashton Kutcher
45 The Chameleon (T.U.F.F. Puppy)
46 Kim Kardashian
Kimberly Noel "Kim" Kardashian West is an American reality television personality, actress, socialite, businesswoman and model.
Her Ass Was So Big That Her Car Went Out Of Control
And Since She's Dumber Then A Rock She Didn't Help
47 The Ginyu Force
48 Noah Ringer
49 Dora the Explorer
Dora's mom: Dora how dare you kill Brian Griffin.
Dora's dad: You are so grounded grounded grounded for 7978979849854398545354 years.
Dora's mom: Go to your room now. - Murvine_Taylor
50 Fred Figglehorn